EAPB0203 induces G₂/M cell-cycle arrest in HTLV-I–positive and –negative human T-cell lines. (A) Effects of EAPB0203 on the cell-cycle distribution of CEM, HuT-102, MT-2, and Jurkat. EAPB0203-treated cells were stained with PI (50 μg/mL), and the cell-cycle analysis was performed using a FACScan flow cytometer. (B) The pre-G₁ percentage represents apoptotic cells. (C) Cycling cells, the sum of (S + G₂/M) phases, are a percentage of nonapoptotic cells. The results are representative of 2 independent experiments.