Conservation of amino acids in a small region of TIN2 between different species. Close-up of the residues mutated in the patients with DC. Arrows indicate the sites of mutations. Changes are K280X (n = 2), R282C (n = 7), R282H (n = 14), P283A, P283H, P283S, T284A, T284Hfs8X, L287P, P289S, F290LfsX2, R291G, and Q298RfsX19. Red indicates highly conserved; blue, limited conservation; black, no conservation. Alignment obtained using MultAlin.²⁰