Evaluation of CD18 expression in neutrophils of miR-CD18 animals. (A) Mouse CD18, CD11b, and CD11a expression was analyzed by flow cytometry in peripheral blood neutrophils (PBNs) in miR-CD18 #85-Tg^hi (solid line), #88-Tg^int (solid line), and wild-type C57Bl/6 (dotted line) mice. Data using isotype IgG are shown as shaded area in histograms. Efficiency of CD18 knockdown was determined using the average geometric mean fluorescence intensity (MFI) plus or minus SEM (graph, bottom) (n = 7-9 for each strain). Dotted line indicates average MFI in CD18-null mice (n = 4). *P < .001. (B) Mouse CD16/32 (left) and CD162 (right) expression on PBNs in #85-Tg^hi and wild-type mice and (C) mouse CD18 and CD11b expression in PBNs isolated from wild-type and #85-Tg^hi mice and stimulated in vitro without (dotted line) or with PMA (solid line) are shown. The MFI of each sample is also given. (D) Analysis of mouse CD18, CD11b, and CD11a on bone marrow neutrophils (BMNs). The MFIs of miR-CD18 #85-Tg^hi mouse (solid line), wild-type mouse (dotted line), and isotype control (shaded area) samples are indicated.