Intracellular localization of DMT1. (A) Confocal microscopy shows that DMT1-II colocalizes with γ-adaptin (trans-Golgi network) and both Ndfip1 and Ndfip2. (B) DMT1-II only partly colocalizes with GM130 (cis-Golgi) and early endosomal marker Rab5. Colocalization is observed with late and recycling endosomal markers Rab7 and Rab11, respectively. Scale bars represent 10 μm.