Phagocytosis and ROS production are reduced in macFOXP1tg macrophages. (A) Phagocytic capability of macrophages from wild-type and macFoxp1tg mice was measured using Vybrant Phagocytosis Assay Kit, as described in “Phagocytosis assay.” Phagocytosis of fluorescein-labeled E coli K-12 BioParticles was quantified by measuring intracellular fluorescence emitted by engulfed particles. Extracellular fluorescence was quenched by trypan blue. NIH 3T3-L1 fibroblasts were included as nonphagocytic control. Data represent mean plus or minus SD from 4 to 5 individual mice per genotype. (B) Superoxide production was detected using a standard assay involving the reduction of cytochrome c in the presence and absence of superoxide dismutase. Data represent the mean plus or minus SD from macrophages isolated from 5 mice per genotype. P value represents 2-way ANOVA.