Figure 1
Figure 1. Polyacrylamide gel electrophoresis of the amplified 5′ parts and 3′ parts of PROCR cDNA derived from HEK293 and EA.hy 926 cells, and of the amplified 3′ part derived from HUVECs from umbilical cords with different PROCR genotypes. (A) Schematic representation of the EPCR cDNA structure and the location of the primers used to amplify the 5′ and 3′ parts. The translated region of the full-length mRNA is in grey. (B) PCR product from HEK 293 EPCR cDNA and EA.hy 926 EPCR cDNA obtained using EPCR2347Fr and EPCR6970Rv as amplification primers. (C) PCR product from HEK 293 EPCR cDNA and EA.hy 926 EPCR cDNA obtained using EPCR4993Fr and EPCR7320Rv as amplification primers. (D) PCR product from HUVECs EPCR cDNA obtained using EPCR4993Fr and EPCR7320Rv as amplification primers.

Polyacrylamide gel electrophoresis of the amplified 5′ parts and 3′ parts of PROCR cDNA derived from HEK293 and EA.hy 926 cells, and of the amplified 3′ part derived from HUVECs from umbilical cords with different PROCR genotypes. (A) Schematic representation of the EPCR cDNA structure and the location of the primers used to amplify the 5′ and 3′ parts. The translated region of the full-length mRNA is in grey. (B) PCR product from HEK 293 EPCR cDNA and EA.hy 926 EPCR cDNA obtained using EPCR2347Fr and EPCR6970Rv as amplification primers. (C) PCR product from HEK 293 EPCR cDNA and EA.hy 926 EPCR cDNA obtained using EPCR4993Fr and EPCR7320Rv as amplification primers. (D) PCR product from HUVECs EPCR cDNA obtained using EPCR4993Fr and EPCR7320Rv as amplification primers.

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