Immunophenotype of BM and spleen cells from FLT3^wt/ITDmice shows expansion of granulocytes /monocytes and DCs and inhibition of B-lymphoid development. (A,E) Increased Lin^−/low and c-KIT⁺ population. (B,F) Increased granulocytic/monocytic and reduced erythroid populations. (C,G,I) Reduced B-cell populations; analyses of total BM/spleen cells (top panels) and B220⁺CD43⁻ BM/spleen cells (bottom panels) are shown. (D,H) Increased DC production. Flow cytometric analysis of spleen and BM cells from representative 1-, 2-, and 12-month-old wild-type and FLT3^wt/ITD mice is shown. Numbers indicate the percentage of cells in each quadrant.