Spleen and BM from FLT3^wt/ITDdisplay MPD. (A-F) Spleen sections from 2-month-old wild-type (A) and FLT3^wt/ITD (B) mice and from 12-month-old wild-type (C,E) and FLT3^wt/ITD (D,F) mice. (G-J) BM sections from 12-month-old wild-type (G,I) and FLT3^wt/ITD mice (H,J). (K,L) Cytospins of BM from 12-month-old wild-type (K) and FLT3^wt/ITD (L) mice. (A-J) H&E stain. (K,L) Wright & Giemsa stain. Scale bars: 100 μm (A,B); 50 μm (C,D,G,H); and 10 μm (E,F,I,J). Image acquisition: Images A-J were acquired at room temperature using a Zeiss Axioskop upright microscope system (Zeiss, Thornwood, NY) with Achroplan 5×/0.16 NA, 10×/0.3 NA, and 40×/0.6 NA objectives. The images were photographed with an AxioCam camera (Zeiss) and Axiovision 4.0 software (Zeiss). Images K and L were acquired at room temperature using a Nikon Eclipse E600 microscope system (Nikon, Tokyo, Japan) with a Nikon 100×/0.90 NA oil objective (magnification: ×1000) and were photographed with a Nikon DMX 1200 digital camera with ACT-1 2.0 software (Nikon).