Phospho-p38 MAPK expression by spleen, PLN, and BM CD8 cells. Membrane and intracellular staining of spleen, PLN, and BM CD8 cells were performed as in Figure 5, except that anti-p38 (T180/Y182) mAb was used. (A) Phospho-p38 staining profiles. Representative staining profiles of spleen and BM samples. As a control, spleen cells were incubated for 15 minutes at 37°C with either medium or LPS at 1 μg/mL and CD11b⁺ cells were analyzed. The scale on x-axis is logarithmic, in arbitrary units, and that on y-axis is linear (maximum value 60 for CD8 and 30 for CD11b panels). The numbers represent the mean fluorescence intensity of phospho-p38 mAb (–) and control mAb (–). (B) Phospho-p38 mean fluorescence intensity. Phospho-p38 mean fluorescence intensity values of spleen, PLN, and BM CD8 cells from individual mice and averages are shown in the left panel. In vitro stimulated spleen CD11b cells from individual experiments and averages are shown in the right panel. Average values are shown by the horizontal bar. The panels summarize the results obtained in 6 independent experiments.