Phenotype of CD8 T cells as a function of CD11c-driven IL-15Rα expression. Lymphocytes from thymus, spleen (SP), lymph node (LN), lung (LG), and bone marrow (BM) were isolated from the mice indicated (6-8 weeks old) and stained for cell-surface markers to identify differences in T-cell populations. (A) Representative flow cytometry plots from thymus and spleen. (B) The percentage of CD8 T cells along with the proportion of naive (CD44^low) and memory-phenotype (CD44^hi) in various tissues. Results are averages with n = 10 mice/group for Tg-1. For Tg-2, n = 8 for spleen and n = 2 for other tissues. § represents a significant difference between naive T cells in Tg and Wt (P < .05), whereas * indicates differences between memory phenotype in Tg and Wt (P < .05). (C) The number of each IEL population recovered from small intestine of the indicated mice. Error bars represent standard deviation (SD) from a representative experiment; values from Tg mice are not significantly different than those from IL-15Rα^−/− mice. (D) Total numbers of myeloid populations in the spleen of the indicated mice. Error bars represent SD, n = 2, for one representative experiment.