Effect of anti-JAM-A mAb (BV-11) on leukocyte responses in LTB4- and IL-1β-stimulated murine cremasteric venules as observed by intravital microscopy. (A) WT mice were given an intravenous injection of saline (control) or of JAM-A mAb or an isotype-matched control mAb (both at a dose of 3 mg/kg); 15 minutes later, the cremaster muscle was exteriorized for observation by IVM. After taking baseline readings of leukocyte adhesion (left panel) and transmigration (right panel), the cremaster muscle was superperfused with Tyrode solution (control) or with a solution of LTB4 (10−7 M in Tyrode). Leukocyte responses of adhesion and transmigration in selected venules were recorded at 10-minute intervals for a duration of 60 minutes. Results are presented as means (± SEM) for n = 4-8 mice/group. (B) WT mice were given an intravenous injection of saline (control) or of JAM-A mAbs or an isotype-matched control mAb (both at a dose of 3 mg/kg) 15 minutes before intrascrotal injection of IL-1β (50 ng/mouse in 400 μL saline). Control mice received intrascrotal injection of saline. At 4 hours later, the mice were prepared for IVM, and leukocyte responses of firm adhesion (left panel) and transmigration (right panel) were quantified. Results are presented as means (± SEM) for n = 4-13 mice/group. Statistically significant differences between control and stimulated groups are shown by asterisks (*P < .05, **P < .01, and ***P < .001), and additional statistical comparisons are indicated by lines and number signs (###P < .001).
