Effects of ω-3 PUFAs on MMP-9 induction in ECs. HUVECs were treated with the combination of VEGF and bFGF (VF) and AA, SDA, DHA, EPA (10 μM), or indomethacin (IND; 5 μM). MMP levels were analyzed by semiquantitative zymography (“Methods”). (A) Differences in molecular weight between pro–MMP-9 standard (92 kDa) and the active MMP-9 (87 kDa) detected in HUVECs (C indicates absence of VF). (B) Representative zymogram of treated HUVEC medium. (C) MMP-9 levels were determined (n = 4) and analyzed statistically. **P < .001 different from VF treatment alone.