ω-3 PUFAs suppress Ang2 induction. (A) Determination of subtoxic PUFA concentrations. Confluent HUVECs were treated with increasing concentrations of PUFAs as indicated for 20 hours. Viability was determined by MTT assay: ● indicates AA; ○, EPA; ▾ DHA; and □, SDA. (B,C) HUVECs were treated with VEGF and bFGF (VF) in combination with PUFAs (10 μM) or indomethacin (IND; 5 μM). Levels of Ang2 were determined by Western blotting (α-tubulin levels are shown to indicate equal loading; C indicates VF absent; n = 3). The intensity of the Ang2 bands was determined by autoradiography and densitometry. Mean differences in Ang2 levels were analyzed statistically. **P < .01; ***P < .001 different from VF alone.