Figure 6
Figure 6. The protein level of p73 is increased by coexpression of YAP2 and wild-type Kpm/Lats2. Any one of HA-tagged Kpm-wild type (wt), Kpm-kinase dead (kd), or mock was cotransfected with TAp73α and any one of FLAG-tagged YAP2-wt, YAP2-S127A, YAP2-1WW*, YAP2-2WW*, or mock into 293T cells by FuGENE-HD reagent. Four rows of lanes from the top in this order represent Western blotting with anti-HA, anti-FLAG, anti-p73, and antiactin in cell lysates. Two independent experiments were performed and representative data are shown.

The protein level of p73 is increased by coexpression of YAP2 and wild-type Kpm/Lats2. Any one of HA-tagged Kpm-wild type (wt), Kpm-kinase dead (kd), or mock was cotransfected with TAp73α and any one of FLAG-tagged YAP2-wt, YAP2-S127A, YAP2-1WW*, YAP2-2WW*, or mock into 293T cells by FuGENE-HD reagent. Four rows of lanes from the top in this order represent Western blotting with anti-HA, anti-FLAG, anti-p73, and antiactin in cell lysates. Two independent experiments were performed and representative data are shown.

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