Induction of p21WAF1 and PUMA but not the IAP family members or bcl-2 is repressed in Kpm/Lats2-knockdown KG-1a cells after treatment with DNA damage–inducing agents. Total mRNA was isolated from Kpm/Lats2-knockdown KG-1a or control cells treated with or without 0.03 μg/mL ETP at day 0 and cultured for 3 days. mRNA expression levels of p21 and PUMA (A), and the IAP family members and bcl-2 (B), were measured by real-time PCR analysis using the specific primers described in Table 1. Data normalized to gapdh are shown as mean plus or minus SD in scale that the value for KG-1a wild-type cells without treatments is 1. Normalization to hprt gave similar results. The analyses were performed in triplicate independently twice, and representative data are shown (Student t test: *P < .05; **P < .005).
