Quantitative analysis of Kpm/Lats2 mRNA in clinical samples and cell lines. (A) The amount of Kpm/Lats2 mRNA was measured in ATL cell lines, ATL clinical samples, normal PBMCs, and normal CD4⁺ T cells. Data normalized to hprt are shown representatively in scale that the value for normal PBMC is 1. Normalization to gapdh gave similar results. The highest one among the ATL cell lines represents ED-40515⁺. Analyses were performed in duplicate independently 3 times and representative data are shown (Welch t test: *P < .001; **P < .005). (B) The amount of Kpm/Lats2 mRNA was measured in NK cell line (YT), NK cell leukemia clinical samples, normal PBMCs (lanes 1-2), and normal CD3⁻56⁺ NK cells (lanes 3-4), NK cell line (YT; lane 5), and NK cell leukemia clinical samples (lane 6-12). Data normalized to gapdh are shown as mean plus or minus SD in scale that the value for normal PBMCs (lane1) is 1. Normalization to hprt gave similar results. Analyses were performed in triplicate independently twice, and representative data are shown.