Cytokine release is not affected in VAMP-8–deficient BMMCs. VAMP-8^+/+ and VAMP-8^−/− BMMCs were sensitized overnight with anti-DNP IgE and stimulated with 10 ng/mL DNP-HSA for the indicated times. Supernatants were collected, and TNF, IL-6, and MIP-1α protein concentrations were determined by ELISA. Data are means plus or minus SEM from triplicate samples derived from 3 different BMMC cultures and are representative of 5 independent experiments.