DC migration is induced by CC chemokines. (A) Chemokines secreted in the cocultures of DC plus T cells with or without TSST1 are quantified by cytometric bead arrays. Each dot represents one donor; mean of chemokine secretion is indicated in each column by the horizontal bar. (B) CCI inhibits DC migration. DCs were pretreated with different concentrations of CCI and migration toward the active supernatant was analyzed. Percentages (mean ± SD) of neutralization of DC migration is calculated as follows: 100 − ((% of CCI-treated DCs in lower chamber/ % of untreated DCs in the lower chamber) × 100). (C) Percentage of DCs attracted toward different recombinant chemokines in transwell chambers are shown. Means plus or minus SD of triplicates from one representative experiment are shown.