The cellular immune response to GFP in SCID mice injected with LV-CMV-GFP previously immune-reconstituted. C57BL/6-SCID mice were immune-reconstituted with 16.5 × 10⁶wt T cells (wt CD90⁺) and 33.5 × 10⁶wt APCs or GFP-tg APCs (wt or GFP-tg CD90⁻) and injected with LV-CMV-GFP the day after. Two weeks after injection, mice were killed and splenocytes analyzed. (A) The presence of GFP⁺ APCs was investigated by FACS within the B-cell (B220⁺CD19⁺) (top dot plots) and the DC (CD11c⁺B220⁻) (bottom dot plots) subsets. One representative dot plot analysis is presented. (B) GFP-specific IFN-γ–producing CD8⁺ T cells present in the spleen were counted by ELISPOT. Data are expressed as average (± SD). One representative experiment of 2 is presented (3 animals per group per experiment).