Comparison between abilities of HIV-1–specific CTLs to suppress HIV-1 replication in CD4+ T cells and macrophages infected with HIV-1 R5 strain. (A) The ability of HIV-1–specific CTL clones to suppress JRFLNL-432 Nef and JRFLNL-M20A Nef replication in CD4+ T cells and macrophages infected with JRFLNL-432 Nef or JRFLNL-M20A Nef. CD4+ T cells and macrophages from HLA-B*5101+ donor were infected with JRFLNL-432 Nef or JRFLNL-M20A Nef and then cocultured with HLA-B*5101-restricted HIV-1–specific CTL clones at various E/T ratios. The amount of HIV-1 p24 antigen in the supernatant on day 9 after infection was measured by using an enzyme immunoassay. (B) Kinetics of JRFLNL-432 Nef and JRFLNL-M20A Nef replication in CD4+ T cells and macrophages infected with JRFLNL-432 Nef or JRFLNL-M20A Nef. The amount of HIV-1 p24 antigen in the supernatant on days 3 to 9 after infection was measured by the enzyme immunoassay. Data shown in the figure are averages of triplicate assays for each time point. The experiments shown in panels A and B were performed simultaneously.
