Three CLL mAb-binding patterns in protein macroarray of human fetal brain recombinant proteins (n = 38 016). Digital images from protein macroarray performed on CII/IGHV1-69_UM/subset-5 mAb, 232B4/IGHV3-48_M mAb, and rIGHV3-21_M/subset-2 mAb. The membranes were incubated with CLL mAbs overnight at + 4°C followed by anti–human IgM/A/G-HRP secondary Ab using Lumiphos substrate for development. The E coli–expressed human fetal brain proteins are positioned in duplicates in specific patterns. Arrows show examples of duplicate positive reactions. Secondary Ab reactivities, nonduplicate, and low-avidity bindings were excluded.