Validation of LEC- and BEC-specific gene expression by quantitative RT-PCR. mRNA expression of LEC- (A) and BEC-specific genes (B), as identified by gene chip analysis, was analyzed in 3 pairs of prospectively prepared samples of LECs and BECs by qRT-PCR. qPCR was performed for the expression of voltage-gated sodium channel type III α (SCN3A) and β chains (SCN3B), insulin-like growth factor 1 (IGF1), platelet-derived growth factor C (PDGFC), glycoprotein M6A (GPM6A), ephrin-A5 (EFNA5), CC chemokine ligand 21 (CCL21, SLC), collectin subfamily member 12 (COLEC12), phosphodiesterase 1A (PDE1A), protein phosphatase 1, regulatory (inhibitor) subunit 9A (PPP1R9A), polycystic kidney and hepatic disease 1-like 1 (PKHD1L1), mesenchyme homeobox 1 (MEOX1), melanoma cell adhesion molecule (MCAM), Duffy blood group antigen (FY), α chain of HLA-DR (HLA-DRA), α chain of HLA-DM (HLA-DMA), MHC class II invariant chain (CD74), EPH receptor A4 (EPHA4), nitric oxide synthase trafficker (NOSTRIN), aquaporin 1 (AQP1), tachykinin receptor 1 (TACR1), VE-cadherin (CD144), and platelet/endothelial cell adhesion molecule (CD31). Data shown are mean mRNA expression values and standard error of means from 2 independent PCR measurements normalized to the expression of β₂-microglobulin (B2M). Asterisks indicate samples in which mRNA levels were below the detection limit of RT-PCR. In each diagram, the mean fold difference of mRNA expression of the 3 paired BEC and LEC samples is given.