Effects of an antirat TNFα antiserum on Ang-II-induced leukocyte accumulation and chemokine mRNA expression and generation in the rat peritoneal cavity. Neutrophil accumulation (A), mononuclear leukocyte accumulation (B), chemokine mRNA expression (C), and the generation of CINC/KC (D), MIP-2 (E), MCP-1 (F), RANTES (G), and MIP-1α (H) in the rat peritoneal cavity. Cell counts and chemokine levels in the peritoneal exudate as well as chemokine mRNA expression in the mesenteric tissue were determined in the following experimental groups: untreated rats intraperitoneally injected with 5 mL PBS, untreated rats exposed to 1 nM Ang-II intraperitoneally, and Ang-II-exposed rats pretreated with the antirat TNFα antiserum (1 mL per rat intravenously) 15 minutes prior to Ang-II intraperitoneally. Results are the mean (± SEM) for 5 to 8 animals per group. *P < .05 or **P < .01 relative to values in the PBS-injected group; + P < .05 or ++ P < .01 relative to the Ang-II untreated group.