Atiprimod-induced signaling and activation of the mitochondrial apoptotic cascades in normal B cells. Western blot analyses showing the protein levels in B cells treated with atiprimod for different times. (A) JNK and phosphorylated JNK (pJNK), Bax, and cleaved PARP (cPARP). (B) Cytosolic (c) and total (t) AIF and cytochrome c. B cells were incubated with 2 μM atiprimod for the indicated time points. Whole-cell lysates or cytosolic fractions were collected and subjected to Western blot analysis. Results of 3 independent experiments are shown.