Atiprimod induces apoptosis of MCL cells. (A) Dose-dependent and (B) time-dependent induction of apoptosis in 4 established MCL cell lines: SP53, MINO, Grant 519, and Jeko-1. (C) Representative histograms of a patient and (D) data from 3 patients showing a dose-dependent induction of apoptosis in freshly isolated primary MCL cells. Cells were treated with different doses of atiprimod for 24 hours and apoptosis was assayed by annexin V–binding and TUNEL assays. Except in Figure 2C, results from TUNEL assay are not shown. Results of 4 independent experiments are shown (mean ± SE).