Effects of atiprimod on the growth of MCL cells and normal PBMCs. (A) Four MCL cell lines: SP53, MINO, Grant 519, and Jeko-1. (B) Freshly isolated primary MCL cells from 4 patients (Pt). (C) Resting PBMCs isolated from 3 healthy volunteers. (D) Activated PBMCs from 3 healthy volunteers by PHA (3 μg/mL), ionomycin (Ion; 1 μg/mL) and PMA (300 ng/mL), or anti-CD3 (5 μg/mL) and anti-CD28 (1 μg/mL) mAbs. Cells were cultured for 48 hours in the presence of 0 to 8 μM atiprimod, with or without the addition of the stimuli. Cell growth was assessed by ³H-thymidine incorporation assay. Results of 3 independent experiments are shown (mean ± SE).