CD40 and c-Rel are expressed in the nucleus of LBCL patient cells. (A) LBCL patient samples (nos. 1-5) were collected as described in “Materials and methods.” Cytosolic and nuclear fractions were extracted and subjected to Western blot analysis. C indicates cytosolic fraction; N, nuclear fraction. (B) Representative patient samples were stained with CD40- and c-Rel–specific antibodies and protein localization was visualized with Cy3 (red) and FITC (green) secondary antibodies under confocal microscope. Nuclear marker Topro-3 (blue) showed the cell nucleus. See “Confocal microscopic analysis” for image acquisition information.