Analysis of early hematopoietic, lymphoid, and erythroid populations in gcsfr morphants. (A-P) WISH analysis of embryos injected with scrambled morpholino (scramMo) or zebrafish GCSFR morpholino 1 (gcsfrMo1) at various time points using antisense probes to specific hematopoietic marker genes, as indicated. (Q-T) The total number of spi1⁺ or c/ebp⁺ cells from embryos injected with either scramMo or gcsfrMo1 were counted, with mean (dashed red line), 95% confidence interval (red lines), and level of statistical significance (*P < .05; **P < .01) indicated.