FACS profiles of CCR3 and CD34 expression on BAL and parenchymal cells, and migration assay results. (A,B) FACS profiles showing granularity (as measured by side light scatter [SSC]) versus CCR3 fluorescence staining on purified cells. (C) Giemsa staining of cytospin preparations from CCR3⁺ sorted cells. Eosinophils (CCR3⁺CD3⁻B220⁻) were sorted from total BAL from OVA-challenged mice. (D,E) Single-color histograms showing CD34 staining intensity on SSC^hi CCR3⁺ gated populations from panels A and B. Gray-filled histogram represents staining intensity from CD34^−/− cells, while the black line represents staining of wt cells. (F) Matrigel migration assay of wt and CD34^−/− sorted eosinophils. The migration index (in response to 30 nM eotaxin) was compared between wt (normalized to 100% migration) and CD34^−/− mice (n=4 independent experiments using pooled BALs cells from 4-6 mice for each experiment; error bar=SEM).