PCI and annexin V are colocalized in atherosclerotic plaques. Cryosections of atherosclerotic plaques were prepared and stained for PCI (monoclonal anti–PCI IgG and Alexa Fluor 568 goat anti–mouse IgG, red) and annexin V (rabbit anti–annexin V IgG and Alexa Fluor 488 goat anti–rabbit IgG, green) as described in “Materials and methods.” Sections were analyzed by confocal laser scanning microscopy. Yellow color indicates colocalization of PCI (red) and annexin V (green). (A) Atherosclerotic plaque type IV-Va, overview; (B) detail corresponding to the framed area in panel A; (C) control without first antibody, same detail as in panel B.