Purification and identification of the ED2 antigen as rat CD163. (A) The approximately 175-kDa ED2 antigen was immunoaffinity purified from rat spleen, electrophoresed, blotted, and stained with Coomassie Brilliant Blue. (B) Analysis of N-linked glycosylation of the CD163/ED2 antigen by endoglycosidase F treatment. (C) SDS-PAGE of CD163/ED2 antigen under reducing (+DTT, 175 kDa) or nonreducing (−DTT, 145 kDa) conditions. All samples were run on 10% SDS-PAGE under reducing conditions (+DTT) unless indicated otherwise.