AQX-016A increases SHIP1 enzyme activity in vitro and in intact cells. (A) Purified pelorol, AQX-016A, 8-epi-pelorol, and 9-epi-pelorol were tested at 2 μM for their ability to enhance SHIP's enzyme activity. (B) The effect of AQX-016A on SHIP1 (♦) and SHIP2 (○) enzyme activity was compared in in vitro enzyme assays. In panels C and D, J16 cells were treated for 30 minutes with 15 μM AQX-016A, 25 μM LY294002, or carrier prior to stimulation with 50 ng/mL of LPS for 15 minutes at 37°C. Cellular lipids were extracted and analyzed for PIP₃ (C) and PI-3,4-P₂ (D) levels as described in “Materials and methods.” Data are expressed as means (± SEM) and are representative of 3 independent experiments.