Figure 6
Figure 6. Expression pattern of HOTAIRM1 during myelopoiesis. HG_U133 transcription profiles of the human HOXA cluster region were assembled for human hematopoietic stem/progenitor cells (HSPC), primary leukocytes, and myeloid cell line NB4. The stem/progenitor cell subpopulations are labeled according to the presence (+) or absence (−) of lineage development markers (CD33, CD34, CD38, or Lin) and low or high rhodamine 123 retention (Rhlow, Rhhigh). Also indicated are the sources of the stem/progenitor cells: bone marrow (BM), cord blood (CB), fetal blood (FB), and G-CSF–mobilized peripheral blood (MPB). NB4 cells were examined either without treatment or after incubation with ATRA and TPA, as indicated. Primary leukocyte populations included total monocytes (MCs), adherent monocytes, monocyte-derived dendritic cells (DCs), resting neutrophils (PMNs), and neutrophils incubated with lipopolysaccharide (10 ng/mL) for 30 or 120 minutes, as indicated. The bar plot presents means and SDs for HOTAIRM1 and HOXA1 transcript levels in each sample group.

Expression pattern of HOTAIRM1 during myelopoiesis. HG_U133 transcription profiles of the human HOXA cluster region were assembled for human hematopoietic stem/progenitor cells (HSPC), primary leukocytes, and myeloid cell line NB4. The stem/progenitor cell subpopulations are labeled according to the presence (+) or absence (−) of lineage development markers (CD33, CD34, CD38, or Lin) and low or high rhodamine 123 retention (Rhlow, Rhhigh). Also indicated are the sources of the stem/progenitor cells: bone marrow (BM), cord blood (CB), fetal blood (FB), and G-CSF–mobilized peripheral blood (MPB). NB4 cells were examined either without treatment or after incubation with ATRA and TPA, as indicated. Primary leukocyte populations included total monocytes (MCs), adherent monocytes, monocyte-derived dendritic cells (DCs), resting neutrophils (PMNs), and neutrophils incubated with lipopolysaccharide (10 ng/mL) for 30 or 120 minutes, as indicated. The bar plot presents means and SDs for HOTAIRM1 and HOXA1 transcript levels in each sample group.

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