Tissue distribution of HOTAIRM1 expression. (A) Multiplex RT-PCR analysis of HOTAIRM1 and internal reference GAPDH transcripts on a multiple tissue human cDNA panel, with each tissue type indicated above the image of a representative agarose gel. (B) Multiplex RT-PCR analysis of HOTAIRM1 and internal reference TUBA1B (101 bp) transcripts on a multiple cell line panel, with each cell type and its treatment with ATRA (− indicates untreated; + indicates treated with 1 μM ATRA for 1 day) shown above the image of a representative agarose gel. Vertical line indicates the location of redundant lanes cropped from the gel image.