Figure 3
![Figure 3. ChIP assay of RNAP II binding to the HOTAIRM1 gene. The 5′ and 3′ ends of the HOTAIRM1 gene (HOTAIRM1-5 [557 bp] and HOTAIRM1-3 [267 bp], respectively), along with 3′ end of the α-tubulin (TUBA1B) gene (484 bp) and the distal U6 snRNA gene promoter (118 bp), were amplified from anti-RNAP II pull-downs and from input DNA of NB4, ATRA induced NB4, and HeLa cells. (Top panel) Agarose gel electrophoresis of multiplex PCR products of the indicated DNA species and cell types. (Bottom panels) Positions of the U6 snRNA and α-tubulin amplicons are shown schematically as shaded bars. (The HOTAIRM1 amplicons are illustrated in Figure 2.)](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/113/11/10.1182_blood-2008-06-162164/5/zh80140933010003.jpeg?Expires=1724237005&Signature=qFbtohRmI-A1MgoSwkdhli7EQGolDVjgyMrRMlMoZrrc1IBmo2uy9KZeU8t2udewXlada5dHmqcp3MIRj6QebTaZO04962Evc9Qb71GVkD9XwzU5AxMtVIss3yyuVY7zdfjL8D7T1~Rv6t5IfaCvGhb7yxfi89~nQVAqAwJkYno66Mw9cgoN37Q2rc114cm7px7FRs0~B-ug781PCntlzAz3o8NHCMEoK1YtJMJEBCuly3agmQBFlcSitTm4R5MlLIjEFs22d6L8xXrgSHMGOg8uzDxCe1JBbepiblJMMv40usuYYNpbMhq-1-SYulDvf-sZPSssQvPcMjeoFakozw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
ChIP assay of RNAP II binding to the HOTAIRM1 gene. The 5′ and 3′ ends of the HOTAIRM1 gene (HOTAIRM1-5 [557 bp] and HOTAIRM1-3 [267 bp], respectively), along with 3′ end of the α-tubulin (TUBA1B) gene (484 bp) and the distal U6 snRNA gene promoter (118 bp), were amplified from anti-RNAP II pull-downs and from input DNA of NB4, ATRA induced NB4, and HeLa cells. (Top panel) Agarose gel electrophoresis of multiplex PCR products of the indicated DNA species and cell types. (Bottom panels) Positions of the U6 snRNA and α-tubulin amplicons are shown schematically as shaded bars. (The HOTAIRM1 amplicons are illustrated in Figure 2.)
