Detection of FLT3/TKD mutations. (A) dHPLC chromatograms of a 278 bp PCR product generated from genomic DNA showing the WT homoduplex and heteroduplex patterns from higher-level D835Y, D835H, ΔI836, and D835V mutations and a lower-level D835Y mutant. (B) Corresponding mismatch PCR and specific restriction enzyme analysis confirming the mutation. W indicates wild-type; M, mutant.