Vector design and experimental setup. (A) Design of retroviral vectors. The backbone in both vectors contains SFFV LTRs and a wPRE. The upper vector contains murine tcirg1 cDNA followed by an IRES, and GFP and is denoted the tcirg1 vector. The lower vector control vector contains an IRES sequence followed by GFP. (B) Northern blot showing mRNA expression of tcirg1 in the transduced cell line 3T3. (C) FL harvest, Ter119 depletion, in vitro transduction, and analysis. (D) Genotyping, irradiation, and transplantation of oc/oc mice.