CD1d expression in myeloma cell lines. Expression of CD1d in 4 myeloma cell lines by: (A) flow cytometry (dotted line represents isotype; continuous line, CD1d) and (B) RT-PCR. G6PD is shown as an amplification control. (C) FISH analysis was used to identify genomic deletions encompassing CD1d in MCL using as a probe a BAC clone spanning the CD1d gene locus. KMS11 is shown here; similar results were seen in another 3 MCL.