Residence of B cells in the peritoneal cavity influences their expression of homing molecules. Ly5.1⁺ splenocytes were injected into congenic Ly5.2⁺ wild-type recipients either intravenously or intraperitoneally 2 days after adoptive transfer, and cells were isolated from the recipients' spleens in case of intravenously transferred mice or from the peritoneal cavities in case of intraperitoneally transferred mice. (A) Surface expression of different chemokine receptors and adhesion molecules of transferred splenic B2 cells was analyzed by flow cytometry. Solid lines show expression on intravenously transferred cells reisolated from the recipients' spleens, dashed lines show expression on intraperitoneally transferred cells reisolated from the recipients' peritoneal cavities, and shaded areas show isotype control stainings for intravenously transferred cells reisolated from the recipients' spleen. Numbers indicate the ratio of the mean fluorescence intensity and the isotype control staining. (B) Adoptively transferred splenic B2 cells reisolated from the spleen do not differ from endogeneous B2 cells in their CXCR5 expression. Expression of CXCR5 was compared for intravenously transferred and endogeneous (Ly5.1⁻B220⁺CD23^highCD21^int) splenocytes. In the middle panels, numbers indicate the percentages of cells within the corresponding gates. In the right panels, numbers indicate the ratio of the mean fluorescence intensity for CXCR5 staining and the corresponding isotype control staining. Shaded areas show isotype control stainings. Representative data for 3 independent experiments with cells pooled from 4 to 8 mice each are shown.