Expression of cathepsin G, lysozyme, and HNP in neutrophils of SCN patients during treatment with G-CSF. Cell lysates of neutrophils purified from patients 1, 2, and 3 (P1, P2, and P3), a healthy control subject (C1), and 2 idiopathic neutropenia patients (C3 and C4) were subjected to Western blot analysis with antibodies raised against neutrophil cathepsin G, lysozyme, HNP precursor, and HNP mature protein. β-Actin was used to normalize protein levels.