Figure 4
Figure 4. Comparative biodistributions using all 3 conjugates in combination or with each conjugate singly at 24 hours. For the combination studies, mice bearing Ramos (A), Raji (B), or FL-18 (C) tumor xenografts were injected intravenously with a cocktail of either 0.47 or 1.4 nmol each of 1F5/SA, Lym-1/SA, and HD39/SA followed 24 hours later with 5.8 or 17.4 nmol, respectively, of CA and 3 hours after that with 1.2 nmol 111In-DOTA-biotin. Mice were killed 24 and 48 hours after injection of 111In-DOTA-biotin and tumor and organs were harvested and analyzed as for the studies in Figure 3. Each graph shows %ID/g 24 hours after injecting 1.4 nmol 1F5/SA alone (□), Lym-1/SA alone (▩), HD39 alone (■), and all 3 used in combination at 0.47 nmol/conjugate (▤) and at 1.4 nmol/conjugate (▧).

Comparative biodistributions using all 3 conjugates in combination or with each conjugate singly at 24 hours. For the combination studies, mice bearing Ramos (A), Raji (B), or FL-18 (C) tumor xenografts were injected intravenously with a cocktail of either 0.47 or 1.4 nmol each of 1F5/SA, Lym-1/SA, and HD39/SA followed 24 hours later with 5.8 or 17.4 nmol, respectively, of CA and 3 hours after that with 1.2 nmol 111In-DOTA-biotin. Mice were killed 24 and 48 hours after injection of 111In-DOTA-biotin and tumor and organs were harvested and analyzed as for the studies in Figure 3. Each graph shows %ID/g 24 hours after injecting 1.4 nmol 1F5/SA alone (□), Lym-1/SA alone (▩), HD39 alone (■), and all 3 used in combination at 0.47 nmol/conjugate (▤) and at 1.4 nmol/conjugate (▧).

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