Analyses of lymphocyte populations in DO11.10-tg RAG-2^−/− mice as a function of time after administration of AAV-EF1α-ova. Examined tissues were spleens and thymus. Shown is the summary of percent CD25⁺ (A,D), CD25⁺GITR⁺ (B,E), and CD25⁺CTLA4⁺ (C,F) of total CD4⁺ cells derived from spleen or thymus. Note that more than 99% of CD4⁺ cells in these mice express ova-specific TCR DO11.10 and are therefore KJ1-26⁺. Antibody staining was carried out using FITC-conjugated KJ1-26 or CD4, PE-conjugated GITR or CTLA4, APC-conjugated CD25. Results are average ± SD for n = 3 mice. Also shown is a summary of percent GITR⁺ (G,H), and CTLA4⁺ (I,J) of CD4⁺CD25⁺ cells from spleen (G,I) or thymus (H,J) as a function of time after vector administration.