Analysis of γ-carboxylase expression. (A) Western blot analysis of mouse fetal liver microsomes probed with an antibody raised against recombinant bovine γ-carboxylase. All lanes were loaded with 5 ng of microsomal protein except for the 2x −/− lane, which contains 10 ng of microsomal protein. (B) Incorporation of ¹⁴CO₂ into FLEEL by solubilized γ-carboxylase from the livers of Ggcx^+/+ mice was considered 100% γ-carboxylase activity, and the incorporation of ¹⁴CO₂ by solubilized carboxylase from Ggcx^+/− and Ggcx^−/− mice were expressed as a percentage of the wild-type. Mean values; error bars indicate ± SD.