American Society of Hematology

Figure 2

$Figure 2. Contrasted susceptibility of target cells to soluble Tag7-Hsp70 and to FasL/Fas-mediated contact killing. (A) K562 cells were incubated for 18 hours with various concentrations of the in vitro assembled 1:1 complex of recombinant Tag7 and Hsp70 (■) and the analogous complex secreted by LAK cells (▩); cytotoxicity of the same recombinant complex toward L929 cells (□) is shown for comparison. (B) Cytotoxicity of CTLs (6-day LAK culture) and their FasL+ and FasL− fractions toward K-562 cells and their Fas+ and Fas− fractions (all separations with antibody-coated magnetic beads), coincubation for 3 hours at 37°C. All data are presented as means (± SEM) in at least 5 independent assays.$

Contrasted susceptibility of target cells to soluble Tag7-Hsp70 and to FasL/Fas-mediated contact killing. (A) K562 cells were incubated for 18 hours with various concentrations of the in vitro assembled 1:1 complex of recombinant Tag7 and Hsp70 (■) and the analogous complex secreted by LAK cells (▩); cytotoxicity of the same recombinant complex toward L929 cells (□) is shown for comparison. (B) Cytotoxicity of CTLs (6-day LAK culture) and their FasL⁺ and FasL⁻ fractions toward K-562 cells and their Fas⁺ and Fas⁻ fractions (all separations with antibody-coated magnetic beads), coincubation for 3 hours at 37°C. All data are presented as means (± SEM) in at least 5 independent assays.

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