Localization of CD53, CD62L, CD63 and CD71 in dividing CD34⁺CD133⁺ cells. (A) Cell-surface distribution of CD53, CD62L, CD63, and CD71 on late mitotic CD34⁺CD133⁺ cells. For each of these markers, 1 mitotic cell is shown containing a symmetric distribution of the given antigen (top row), and 1 containing an asymmetric distribution (bottom row; green staining). Cells are counterstained with an anti-CD133 antibody (red staining) and DAPI (blue staining). Light microscopy images of the stained cells are presented in the fifth column. (B) Intracellular distribution of CD63 (red staining) and CD71 (green staining). The overlay of the 2 is given in the third column.