STAT5 silencing enhances HIV RNA expression in IL-2 stimulated CD8-depleted PBMC of HIV-positive individuals. (A) Western blot analysis of WCE of PBMC from individual number 8 (patient no. 8) after electroporation with either control or STAT5 siRNA. Membranes were sequentially hybridized with anti-STAT5 mAb (upper panel) and a control anti-α-actin Ab (lower panel). (B). CD8-depleted PBMC from patient nos. 9 and 10 were electroporated with either control or STAT5 siRNA; 24 hours later, the cells were resuspended in a medium enriched with IL-2 and intracellular HIV RNA expression was analyzed by flow cytometry after 4 days of cultivation; gray thick histograms: scramble control siRNA; black thick histograms: STAT5 siRNA.