shRNA reduces syndecan-1 expression on myeloma cells. (A) Expression of syndecan-1 mRNA by lentiviral-infected CAG cells was quantified by PCR (lane 1, control shRNA cells; lane 2, syndecan-1 shRNA cells). Bottom panel shows GAPDH control. Bands migrated to their predicted location relative to standards with sizes of 396 bp for syndecan-1 and 452 bp for GAPDH, respectively. (B) Flow cytometry showing cell-surface expression of syndecan-1 on shRNA control (open peak) or shRNA syndecan-1 (shaded peak) cells. (C) Western blot for syndecan-1 from CAG wild-type (lane 1), control shRNA (lane 2), and syndecan-1 shRNA cells (lane 3).