PGE₂-dependent expression of costimulatory molecules of the TNF superfamily on MoDCs. (A) Quantitative real-time RT-PCR analysis of TNFSF4 (OX40L), TNFSF7 (CD70), and TNFSF9 (4-1BBL) mRNA expression in MoDCs matured in the absence or presence of PGE₂. Up-regulation of specific mRNA expression levels is depicted as fold increase by PGE₂. Mean values and SEM derived from 7 individual donors are shown. Cell-surface expression of OX40L (B, black bold lines) and CD70 (C, black bold lines) on immature MoDCs (iDC) or sCD40L-matured DCs in the absence or presence of PGE₂ after 24 hours or 48 hours of culture was analyzed by flow cytometry. MoDCs were generated either in serum-free AIM-V medium (A-C) or AIM-V medium containing 5% human AB serum (D). Gray thin lines represent isotype control stainings. A representative of 3 (D) or at least 5 (B,C) independent experiments with different donors is shown.