Figure 2
![Figure 2. Minigene analyses of ankyrin mRNA splicing. Wild-type and mutant ankyrinAnkara minigenes from exon 15 to exon 20 were prepared and transfected into COS and K562 cells. The sequence of the RT-PCR products derived from RNA of transfected cells was analyzed. RNA from cells transfected with the wild-type minigene contained the expected normally sized and spliced product (wild-type [WT], top line). RT-PCR products derived from RNA of cells transfected with the mutant ankyrinAnkara minigene yielded 11 mutant spliceoforms, shown schematically, but no wild-type product. Similar studies with reticulocyte RNA from the proband yielded the same results, except that the +26-bp and +144-bp spliceoforms were not found.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/12/10.1182_blood-2006-09-046573/4/zh80120701240002.jpeg?Expires=1722407982&Signature=FuflwRtCgfzrSOZ6djRw8fkR-mPAADZKPVwxQo2BpttKO6pFeQxdJjUiZZz5KqrWoYiaUBk8w~hapxhNtixmW3kfBQC0TpZN10hNV0Q7iJdENLHJVTExwBcXR0TqlKlyKBfaDaCYDbtce-sPgAe0My57IL~AuRupiGTaxDupI8bxelYsRBCtTAdrjjSv3nEPZTM9JzkVPBgPfdAfx17nVRmGaF84ITDW8R39clDl2khydvD5tBkHpr8eJbt7rN4ymUForfU8Iefu7ymiDUa4tN-L~ubc96RYiRI53huzN4-kUKyeEyr-oDYJyvVqZxwzMZ32moRdj6jMn5n~NkYbtA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Minigene analyses of ankyrin mRNA splicing. Wild-type and mutant ankyrinAnkara minigenes from exon 15 to exon 20 were prepared and transfected into COS and K562 cells. The sequence of the RT-PCR products derived from RNA of transfected cells was analyzed. RNA from cells transfected with the wild-type minigene contained the expected normally sized and spliced product (wild-type [WT], top line). RT-PCR products derived from RNA of cells transfected with the mutant ankyrinAnkara minigene yielded 11 mutant spliceoforms, shown schematically, but no wild-type product. Similar studies with reticulocyte RNA from the proband yielded the same results, except that the +26-bp and +144-bp spliceoforms were not found.
