Figure 5
Figure 5. Q-PCR analysis on transduced AML CD34+ cells. CD34+ cells from AML nos. 2 and 11 were transduced with Venus control vectors or C/EBPα and plated on MS5 for 1 week in the presence of 500 nM 4-OHT, after which GFP+ cells were sorted by MoFlo and RNA was extracted for Q-PCRs as indicated. GAPDH expression was used as internal control, and the expression levels were normalized to 1 in the Venus control groups. Error bars denote the standard deviations, belonging to the normalized means as they are shown. ND indicates not detectable, expression was below detection threshold.

Q-PCR analysis on transduced AML CD34+ cells. CD34+ cells from AML nos. 2 and 11 were transduced with Venus control vectors or C/EBPα and plated on MS5 for 1 week in the presence of 500 nM 4-OHT, after which GFP+ cells were sorted by MoFlo and RNA was extracted for Q-PCRs as indicated. GAPDH expression was used as internal control, and the expression levels were normalized to 1 in the Venus control groups. Error bars denote the standard deviations, belonging to the normalized means as they are shown. ND indicates not detectable, expression was below detection threshold.

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